DNA extraction facility at the Swegene genotyping center in Malmö.

What is offered

The facility provides the necessary infrastructure to efficiently extract DNA and quantify, normalise and store the extracted DNA in a quality-assured manner, accredited by SWEDAC. All samples within Swegene projects will be treated according to the same quality assurance rules as are used in the accredited processing of the clinical diagnostic samples. All lab processes are organized via a LIMS (laboratory information management system) which tracks all samples and all operations performed through the whole analysis process.

Lab processes

• DNA isolation from whole blood and various cell suspensions such as buffy coat is made using Qiagen kits. Two scales are available, mini (200 mikroliter) and maxi (10 ml).
• DNA quantitation and normalization as well as sample reorganization, reformatting and pooling
• Arraying of normalised samples with extracted DNA in bar-coded 96-well plates, linked to a robotic work station that can retrieve samples based on electronic pick-up lists.

Staff

• Agneta Östensson, technician
• Maria Sterner, technician
• Liselotte Hall, technician
• Agneta Heckton-Sterner, technician
• Ylva Johnson, technician
• Stefan Strömberg, technician

Access

The Resource Centre offers services to all Swedish academic researchers who want to study the role of genetic variation in the development of disease. A prerequisite is that the samples have been stored in accordance with bio-banking rules and that the project has been approved by an ethics committee. The steering committee will be consulted for advice on priority issues.

Prices

Malmö:
As a rule, prices are subject to negotiations, as they are heavily dependent on the exact number of samples and analysis systems that are being analyzed. See list below for price estimates for large projects.

 

Contact

Head of Genotyping Unit
Dr. Christer Hallden
E-mail: Christer.Hallden@klkemi.mas.lu.se